WebIt was then reacted with horseradish peroxidase-conjugated secondary antibody (goat anti-rabbit immunoglobulin G (IgG) or goat anti-mouse IgG, 1:10 000 dilution; Jackson). The blotting signals were visualized by Western lighting plus ECL (PerkinElmer) and detected by an ImageQuant TM LAS-4000 mini luminescent image analyzer (GE). WebProtein samples were resolved on a polyacrylamide gel with a 4%–20% gradient (Cosmo Bio Ltd., Tokyo, Japan) and transferred to nitrocellulose membranes (Cytiva). The membranes were probed with first and secondary antibodies, and the specific signals were detected using Western Lightning Plus-ECL (PerkinElmer).
Western Lightning® Plus-ECL, Enhanced ... - SelectScience
Web17. mar 2024 · The bands were detected using Western Lightning Plus-ECL, Chemiluminescent Substrate (Perkin Elmer) and visualized using Amersham ImageQuant 800 (GE Healthcare). In RNA pull-down assays, to enhance the signals, SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific) was added to Western … WebWestern Lightning Chemiluminescence Reagent Plus by PerkinElmer, Inc. Manufacturer PerkinElmer, Inc. Be the first to review this product The Western Lightning™ Chemiluminescence Reagent Plus is a non-radioactive light-emitting system designed to detect proteins immobilized on a membrane. houteicyouso
Involvement of chemerin and CMKLR1 in the progesterone …
WebWestern Lightning ™ Plus Chemiluminescence Reagent is designed for sensitive and consistent detection of proteins immobilized on a membrane. The method provides a … http://citations.perkinelmer.com/search.php?sqlQuery=SELECT%20author%2C%20title%2C%20year%2C%20publication%2C%20volume%2C%20pages%2C%20keywords%20FROM%20refs%20WHERE%20%28title%20RLIKE%20%22Volocity%22%20OR%20abstract%20RLIKE%20%22Volocity%22%20OR%20keywords%20RLIKE%20%22Volocity%22%20OR%20pages%20RLIKE%20%22Volocity%22%20OR%20publication%20RLIKE%20%22Volocity%22%20OR%20area%20RLIKE%20%22Volocity%22%29%20ORDER%20BY%20call_number%20DESC&submit=List&citeStyle=APA&orderBy=call_number%20DESC&showQuery=0&formType=sqlSearch&showRows=10&rowOffset=550&client=&viewType=PE-head Webwere visualized using Western Lightning® Plus-ECL (PerkinElmer). For IHC, Signalstain® boost IHC detection reagent (Cell Signaling Technologies) and anti-rabbit or anti-mouse ... Western blotting pictures were used for densitometric analysis; bands were normalized for equal loading and total protein forms, and subsequently compared to control ... houteicyousho